PhD-candidate Advanced Microscopy for Immunology

We are looking for a PhD-candidate for a research project focused on developing and applying super-resolution light microscopy with cryogenic electron microscopy (CryoEM). Are you the candidate we are looking for?

2 maanden geleden

Arbeidsvoorwaarden

Standplaats:: Albinusdreef, Leiden, Zuid-Holland
Dienstverband:: Tijdelijk contract / Tijdelijke opdracht
Uren per week:: 36 uur

Salarisindicatie:: € 2495 - € 3196 per maand
Opleidingsniveau:: WO

Functieomschrijving

You will join the lab of Thom Sharp to work on developing and applying super-resolution light microscopy correlated with CryoEM (super-res cryoCLEM). We have developed a method to perform super-res cryoCLEM on intact mammalian cells using fluorescent proteins. One aspect of this PhD function is designing and building the next-generation of cryo-super resolution light microscopes, to improve ease of use and explore novel methods for super-res cryoCLEM. This will be performed in collaboration with Lennard Voortman, the head of the LUMC light microscopy facility. Another aspect is to use super-res cryoCLEM to investigate cellular processes, with a focus on the human immune system. Applications include, but are not limited to, in-situ structural biology of antibody binding and effector complexes, phagocytosis, and the biology of antigen processing by MHC complexes.

In short

· You will, in collaboration with the LUMC light microscopy facility, design and build the next generation of cryo-super resolution light microscopes

· You will use super-res cryoCLEM to investigate cellular processes, focusing on the human immune system

· You have a background in microscopy, physics or engineering

Functie-eisen

We prefer a candidate with a background in microscopy, physics or engineering and a relevant MSc degree is required. You have experience in biology/immunology with an evident desire to explore the fundamentals of super-res cryoCLEM.

Conditions

You will be appointed for the duration of four years. Your salary is a maximum of € 2,495 in the first year, amounting to a maximum of € 3,196 in the final year (scale Pro, Collective Labour Agreement University Medical Centers).

Additional information

Your career at the LUMC

The LUMC offers opportunities to maintain and develop your knowledge and skills. We offer internal courses and in-service training to help you with your personal development. We also offer services for mobility and career advice.

Contact details
Thomas Sharp, assistant professor, department of Cell and Chemical Biology, Telephone: +31 (0)71 526 94 99, E-mail: t.sharp@lumc.nl

Acquisition as a result of this vacancy is not appreciated.