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PhD Candidate Epigenetic Changes in Bone Tumours

We are looking for a PhD candidate for a research project focused on the development of mass spectrometry-based tools for the untargeted and in-depth characterization of epigenetic changes of bone tumours, specifically those of histones. Are you the candidate we are looking for?

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Arbeidsvoorwaarden

Standplaats:
Albinusdreef, Leiden, Zuid-Holland
Dienstverband:
Tijdelijk contract / Tijdelijke opdracht
Uren per week:
36 uur
Salarisindicatie:
€ 2495 - € 3196 per maand
Opleidingsniveau:
WO

Functieomschrijving

In this highly multidisciplinary joint PhD project, we aim to develop high resolution mass spectrometry-based methods for the in-depth proteomic characterization of epigenetic heterogeneity and apply these methods to elucidate the role of epigenetic changes in the pathogenesis of bone tumours. You will focus on the in-depth characterization of cell-line derived histone proteoforms using top-down and middle-down proteomics approaches. You will also focus on the characterization of epigenetic and metabolic states upon treatment with epigenetic compounds. Furthermore, you will assess intratumour epigenetic and metabolic heterogeneity of various patient derived bone tumours, using high-resolving power mass spectrometry imaging. Finally, you will focus on the metabolic and epigenetic MSI-based characterization of tumours, obtained from patients enrolled in a clinical trial.

In short

·         You will contribute to research to better understand the biology underlying treatment of bone tumours

·         Specifically, you will work on the untargeted and in-depth characterization of epigenetic changes of bone tumours using mass spectrometry approaches

·         This is a multidisciplinary, joint PhD project, in which you will also spend approximately one year in Pisa, Italy

 

Functie-eisen

You have obtained your MSc degree in analytical chemistry or a biomedical area (or will do so on very short term). You must be willing to spend approximately one year in Pisa, Italy. Furthermore, it is required that you have some experience with (MALDI) mass spectrometry and cell biological techniques, preferably in the field of oncology, and have good technical skills. It would be preferred if you also have experience in computer scripting, and data analysis. You must be fluent in English, performance driven, structured and accurate and have good communication and writing skills.

Conditions

You will be appointed for the duration of four years. Your salary is a maximum of € 2,495 in the first year, amounting to a maximum of € 3,196 in the final year (scale PhD students, Collective Labour Agreement University Medical Centers).

Additional information

Your career at the LUMC

The LUMC helps you with maintaining and developing your knowledge and expertise. We offer internal courses and in-service training to assist you with your personal development. We also offer services in terms of personal development, mobility and career advice.

Project information

Epigenetics govern the interactions between genes and their products that ultimately result in phenotype, and are essential for cell development and maintenance. Epigenetic alterations can lead to changes in gene expression or function, and result in malignant transformation of cells. Therefore, changes in the epigenetic landscape are considered one of the hallmarks of cancer.

Primary bone tumours are rare and as a consequence considered difficult to diagnose by pathologists. More than 50 entities are described in the World Health Organization 2020 classification and there is considerable morphological overlap between separate entities. Treatment options for most bone sarcomas include surgery, chemo-, and radiotherapy. Although effective for some, not all bone tumours (e.g. chondrosarcoma) are sensitive to chemo-/radiotherapy treatments. In order to develop novel diagnostic tools, find new druggable targets, or repurpose therapeutic molecules, the molecular pathways underlying the formation and progression of bone tumours need to be unraveled in greater detail.

References can be requested as part of the procedure. You will be asked to give a presentation.

Read more about the Department of CPM here.

Read more about the research group Bone and Soft Tissue Tumour Pathology here.

Contact details: Bram Heijs, researcher CPM

Telephone: +31(0)71 526 41 54.