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PhD Candidate MS-based Antibody Characterization in Autoimmune Diseases

We are looking for a PhD candidate for a research project focused on the development and application of mass spectrometry (MS) in combination with separation techniques for the structural and functional characterization of antibodies in autoimmune diseases. Are you the candidate we are looking for?

2 maanden geleden

Arbeidsvoorwaarden

Standplaats:
Albinusdreef, Leiden, Zuid-Holland
Dienstverband:
Tijdelijk contract / Tijdelijke opdracht
Uren per week:
36 uur
Salarisindicatie:
€ 2495 - € 3196 per maand
Opleidingsniveau:
WO

Functieomschrijving

In this multidisciplinary PhD project we aim to develop high resolution mass spectrometry-based methods in combination with separation techniques for the comprehensive characterization of disease-associated antibodies. You will work with high-resolution MS and apply intact, top-down and middle-up/down approaches. You will also develop affinity approaches (e.g. affinity capillary electrophoresis-mass spectrometry) to study the differential binding of antibody proteoforms to key receptors. You will apply the techniques to study autoantibodies from patient samples of three autoimmune diseases (systemic lupus erythematosus, rheumatoid arthritis, and ANCA-associated vasculitis) to understand their pathogenicity and find new disease biomarkers.

In short

· You will contribute to unravel antibody diversity and their biological implications under specific disease conditions

· You will work on the structural and functional characterization of autoantibodies derived from patients with systemic lupus erythematosus, ANCA-associated vasculitis and rheumatoid arthritis

· This is a multidisciplinary PhD project in collaboration with the departments of Rheumatology and Nephrology

Functie-eisen

You hold a MSc degree in analytical chemistry, pharmaceutical sciences or a biomedical area (or will do so on short term). You have some experience with analytical techniques such as mass spectrometry, liquid chromatography or capillary electrophoresis preferably for protein analysis, and you have good technical skills. You have an interest and background in protein biochemistry and bioanalytical method development. You have a fascination for clinically relevant analysis in the field of immunology. You are fluent in English, like to work in a team and have good communication and writing skills.

Conditions

You will be appointed for the duration of four years. Your salary is a maximum of € 2,495 in the first year, amounting to a maximum of € 3,196 in the final year (scale Pro, Collective Labour Agreement University Medical Centers).

Additional information

Your career at the LUMC

The LUMC helps you with maintaining and developing your knowledge and expertise. We offer internal courses and in-service training to assist you with your personal development. We also offer services in terms of personal development, mobility and career advice.

Project information

Many autoimmune diseases are characterized by the presence of pathogenic autoantibodies directed towards self-antigens. Systemic lupus erythematosus, ANCA-associated vasculitis and rheumatoid arthritis are some examples. These autoantibodies show a large structural and functional heterogeneity and not all autoantibodies exhibit the same level of pathogenicity. Although known for many years (and in many cases used as biomarkers) there still remain a lot of open questions regarding the structure and function of these autoantibodies.

This project aims to develop mass spectrometry-based analytical platforms in combination with separation techniques to allow detailed characterization of human antibody proteoforms and to understand their capacity to trigger immune responses. To this end, relevant antibody subunits will be analyzed in their intact/native state permitting proteoform characterization. Next to their structural characterization their different capacity to activate the immune response will be investigated by using MS-based affinity approaches (e.g. affinity capillary electrophoresis-mass spectrometry). During this project the developed techniques will be applied to study the proteoforms of autoantibodies present in three autoimmune diseases exhibiting severe inflammatory conditions: systemic lupus erythematosus, rheumatoid arthritis, and ANCA-associated vasculitis. The study will provide pathophysiological insights and contribute to the development of markers with potential in diagnosis and therapy design.

Read more about the CPM here.

Acquisition as a result of this vacancy is not appreciated.

Contact details
Elena Domínguez Vega, Principal Investigator, Center for Proteomics and Metabolomics, Telephone: +31 (0)71 526 69 98, e-mail: e.dominguez_vega@lumc.nl