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Postdoc in Gene Editing and Human iPSC-derived retinal organoids

Do you have a PhD in biomedical sciences and biochemical, molecular cell biological or neurobiological experience? Are you interested in developing novel gene editing therapies for children and adults with retinal diseases? Then we have an interesting postdoc position for you.

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Arbeidsvoorwaarden

Standplaats:
Albinusdreef, Leiden, Zuid-Holland
Dienstverband:
Tijdelijk contract / Tijdelijke opdracht
Uren per week:
36 uur
Salarisindicatie:
€ 4481 per maand
Opleidingsniveau:
WO

Functieomschrijving

We performed gene augmentation therapy studies and tested these on human iPSC-derived retinal organoids as models for human retinal disease. We plan to develop novel methods that allow gene correction by editing in photoreceptors and retinal pigment epithelium. There are a multifold of genes that if mutated cause non-syndromic blindness. Each of these retinal disease genes may become nonfunctional due to various mutations in one or two of the many exons or regulatory sequences of a disease gene. You will develop novel gene editing methods to rescue the loss of gene function due to mutations. You will also use other cellular and molecular techniques (FACS, qPCR, microscopy, immunofluorescence).

Summary of the required skills

·        You hold a PhD in Biomedical Sciences

·        You are interested in the development of novel gene editing therapy in human iPSC-derived retinal organoids

·        You can culture human pluripotent stem cells

·        You will work with state-of-the-art equipment and in an innovative academic environment

 

 

Functie-eisen

We are searching for a highly motivated and experimentally talented scientist with a drive to develop novel therapies for the retina. Preferably, you are familiar with molecular biological research and CRISPR-CAS gene editing therapy on mammalian iPSC-derived organoids. Reporting and publishing the results in high impact scientific journals and at international conferences is expected. We are looking for someone who is flexible and can work well together

Conditions

You will be employed on the basis of 36 hours per week. Appointment is for minimal 1 year. Your salary is based on your education and experience and is a maximum of  € 4,481  gross per month for a full time position (scale 10 in the Collective Labour Agreement for University Hospitals).

Additional information

References

Quinn PM et al. 2019. Human iPSC-derived retinas recapitulate the fetal CRB1 CRB2 complex formation and demonstrate that photoreceptors and Müller glia are targets of AAV5. Stem Cell Reports 12:906-919.

Pellissier LP et al. 2015. Gene Therapy into Photoreceptors and Muller Glial Cells Restores Retinal Structure and Function in CRB1 Retinitis Pigmentosa Mouse Models. Hum Mol Genet 24:3104-3118.

Quinn PM et al. 2017. The CRB1 Complex: Following the Trail of Crumbs to a Feasible Gene Therapy Strategy. Front Neurosci 11:175.

Contact: Jan Wijnholds, associate professor / group leader, Department of Ophthalmology

+31(0)71 526 92 69

j.wijnholds@lumc.nl