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Postdoc in Transcription-coupled DNA Repair

Are you fascinated by how DNA damage is repaired? We are looking for a postdoc to unravel the biochemical basis of transcription-coupled DNA repair in human cells.

28 dagen geleden

Arbeidsvoorwaarden

Standplaats:
Albinusdreef, Leiden, Zuid-Holland
Dienstverband:
Tijdelijk contract / Tijdelijke opdracht
Uren per week:
36 uur
Salarisindicatie:
€ 4481 per maand
Opleidingsniveau:
WO

Functieomschrijving

The presence of DNA damage is a major complication during gene transcription. The transcription-coupled DNA repair (TCR) pathway ensures the efficient removal of transcription-blocking DNA damage from active genes, but the underlying mechanisms are not understood. Work in the Luijsterburg lab is focused on understanding these mechanisms. In the current project, funded by an ENW-klein grant from NWO, you will study the TCR pathway by employing biochemical, proteomics and genetics approaches to reveal how the TCR proteins work together and interact with each other to orchestrate efficient removal of transcription-blocking DNA damage. To this end, you will use CRISPR-Cas9-mediated genome editing, combined with protein cross-linking coupled with mass spectrometry (XL-MS) and proximity-dependent biotin identification (BioID).

Summary of the required skills

·        You have a PhD degree in cell or molecular biology

·        You will work on unravelling mechanisms of DNA repair during gene transcription

·        You have multiple years of experience as a researcher and preferably on the subject of DNA repair

Functie-eisen

You hold a PhD in cell or molecular cell biology. You are highly motivated and have proven research abilities and an excellent publication record. Experience with research on DNA repair mechanisms is an advantage. Excellent skills in molecular biology and cell biology are essential. Ideally, you have expertise in one or more of the following techniques: human cell culture, fluorescence microscopy, proteomics, immunoprecipitation and CRISPR/CAS9-based genome engineering. You are organized, accurate, possess excellent communication skills in English, and you are willing and able to work both independently and also collaboratively as part of a dynamic team.

Conditions

You will be employed on the basis of 36 hours per week. Appointment is for a minimum of 1 year, possibly to be extended to up to another 2 years. Your salary is based on your education and experience and is a maximum of  € 4,481  gross per month for a full time position (scale 10 in the Collective Labour Agreement for University Hospitals).

Additional information

Your career at the LUMC

The LUMC stimulates you in maintaining and developing your knowledge and skills. We offer internal courses andand continuous training as well as services in terms of  mobility, personal development and career advice.

You can find more information on the Luijsterburg lab here.

More information on the cluster Genetic Instability and Cancer cluster and Dr. Martijn Luijsterburg.

Contact: Dr. Martijn Luijsterburg, assistant professor, Human Genetics department, telephone +31 (0)71 526 96 12, email: s.m.luijsterburg@lumc.nl

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