PhD position: Single-cell dynamics within lung organoids

Organoids are miniature organs that can be grown in-vitro from adult stem cells. We have recently found that cells within lung organoids become surprisingly motile upon infection by the RSV virus, resulting in a dramatic ...

13 dagen geleden

Arbeidsvoorwaarden

Standplaats:
Van Vollenhovenlaan, Utrecht, Utrecht
Dienstverband:
Tijdelijk contract / Tijdelijke opdracht
Uren per week:
40 uur
Salarisindicatie:
€ 2834 per maand
Opleidingsniveau:
WO

Functieomschrijving

Organoids are miniature organs that can be grown in-vitro from adult stem cells. We have recently found that cells within lung organoids become surprisingly motile upon infection by the RSV virus, resulting in a dramatic, collective rotation of the entire spherical organoid. The ‘Quantitative Developmental Biology’ research group will use a quantitative, physics-inspired approach to study problems in developmental biology, focusing on the small roundworm C. elegans. The aim of the research is to elucidate how living organisms can reliably build their bodies during development, despite the considerable underlying variability on the molecular level. Organoids are miniature organs that can be grown in-vitro from adult stem cells. We have recently found that cells within lung organoids become surprisingly motile upon infection by the RSV virus, resulting in a dramatic, collective rotation of the entire spherical organoid. The aim of this project is to uncover the responsible mechanisms, which has implication for understanding the fundamentals of coordinated cellular motion in tissues and its role in viral infections. We will study these questions using confocal and light-sheet microscopy, cellular tracking analysis, and mathematical modelling. We will use fluorescent labeling to follow cell movement in space and time, both on the cellular and sub-cellular level. These highly quantitative data will provide a unique insight into the dynamic response of the lung epitheleum to viral infection and collective cell moltility in general. This is a joint project between the Tans and van Zon labs at AMOLF Amsterdam and is in collaboration with the Clevers lab at the Hubrecht institute, and the HUB institute in Utrecht. The 'Biophysics research group' focusses on three themes.

Functie-eisen

We are looking for an outstanding experimental physicist, chemist, or biologist with skills in handling complex data and an interest in fundamental questions about development, and a strong drive to excel in a competitive international environment. Prior experience with quantitative (light-sheet) microscopy, organoid/tissue culture or quantitative analysis of single-cell behavior is not required, but is considered a plus.

Conditions

When fulfilling a PhD position at NWO-I, the Institutes Organisation of NWO, you will get the status of junior scientist.
You will have an employee status and can participate in all the employee benefits NWO-I offers. You will get a contract for four years. Your salary will be up to a maximum of 2,834 euro gross per month. The salary is supplemented with a holiday allowance of 8 percent and an end-of-year bonus of 8.33 percent.
You are supposed to have a thesis finished at the end of your four year term with NWO-I.
A training programme is part of the agreement. You and your supervisor will make up a plan for the additional education and supervising that you specifically need. This plan also defines which teaching activities you will be responsible (up to a maximum of ten percent of your time). The conditions of employment of NWO-I are laid down in the Collective Labour Agreement for Research Centres (Cao-Onderzoekinstellingen), more exclusive information is available at this website under Personeelsinformatie (in Dutch) or under Personnel (in English).
General information about working at NWO-I can be found in the English part of this website under Personnel. The 'Job interview code' applies to this position.

Additional information

Prof.dr.ir Sander Tans (Group leader Biophysics) and Dr. Jeroen van Zon (Group leader Quantitative Developmental Biology). Phone: +31 (0)20-754 7100.

Bedrijfsomschrijving

AMOLF initiates and performs leading fundamental research on the physics of complex forms of matter, and creates new functional materials, in partnership with academia and industry. AMOLF is located at the Amsterdam Science Park, The Netherlands, and engages approximately 140 scientists and 70 support staff. See also www.amolf.nl

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