Project for Academic Biochemist Development of RNA based in-situ hybridization method
PanPath is searching for an academic biochemist for a short time (3-6 month) development project.
- Instraat, Budel, Noord-Brabant
- Uren per week:
- 40 uur
- Aantal jaren werkervaring:
- 0-2 jaar
DNA is made up of bases (nucleic acids). There are 4 bases: cytosine (C), guanine (G), adenine (A) and thymine (T). These bases form long chains (the total human genome comprises approximately 3 billion base pairs), the order and length of the bases determines the properties / functions of a certain chain (i.e. a gene). DNA is located in the cell nucleus. The functional properties are expressed by reading (copying) a sequence; the copy is transported from the cell nucleus to the cytoplasm of the cell where the copy is translated into a functional protein. The copy is called RNA. RNA is an exact copy of the specific coding DNA (the target). Target sequences can be selected on the basis of analyzing DNA databases and can be produced synthetically. These synthetic sequences can be used to identify target DNA by in-situ hybridization techniques. Synthesized RNA sequences are short and contain 10-50 bases.
With in-situ hybridization techniques, the probe must be labeled with a marker to allow detection. With short sequences it is not possible to label enough markers on the probe with a low sensitivity as a result. However, with a large number of target sequences each labeled with polymer detectable labels a sensitivity of 96% can be reached according to recent publications.
a Msc degree in the field of Biochemistry is required