3 dagen geleden - Radboud Universiteit (RU) - Nijmegen
PhD candidate 'linking mTOR to deregulated GABA signalling in human IPSC-derived network models for neurodevelopmental epilepsy'
This PhD candidate position is available for a fixed term of 4 years to perform research in relation to mTORpathies and neurodevelopmental epilepsy at the …
- Geert Grooteplein Zuid, Nijmegen, Gelderland
- Tijdelijk contract / Tijdelijke opdracht
- Uren per week:
- 36 - 36 uur
- € 2495 - € 3196 per maand
This PhD candidate position is available for a fixed term of 4 years to perform research in relation to mTORpathies and neurodevelopmental epilepsy at the Cognitive Neuroscience Department at the Radboudumc in Nijmegen. Epilepsy is a common neurological disease, affecting approximately 180,000 people in the Netherlands and treating childhood-onset genetic epilepsies remains challenging. Our overall aim is to gain mechanistic insight into the mTOR-dependent regulation of GABAergic networks in developmental epilepsies. We developed an in vitro model to investigate neuronal development, by differentiating hiPSCs into excitatory neurons (iNeurons) and forebrain organoids. We will use patient-derived hiPSCs CRIPSR/Cas9-edited lines carrying mTORpathy related mutations as well as TSC2 patients derived lines.
You will perform molecular (transcriptomic), structural (immunohistochemistry & quantitative morphometry) and functional phenotyping aiming for unravelling common molecular and cellular mechanisms of mTORpathies and developmental epilepsy. The functional phenotyping will employ measurements using state or the art high-density microelectrode arrays (MEAs) on 2-dimensional neuronal networks as well as on early developmental stages of brain organoids.
The project is part of an interdisciplinary collaboration with the Pathology Department at the Amsterdam UMC (E. Aronica group), connecting researchers with expertise in epilepsy, neuropathology and neurophysiology to elucidate the mechanisms underlying altered neuronal network function in epilepsy. The research will be located at the Cognitive Neuroscience Department (D. Schubert group) in strong collaboration with the Human Genetics department (N. Nadif Kasri group) and embedded in the Donders Institute for Brain, Cognition and Behaviour of the Radboud University Medical Center.
Our research team at the Radboud university medical center is renowned for its genetic, molecular and electrophysiological research in the field of neurological and neurodevelopmental disorders. Over the past years we have developed unique knowledge and resources, such as the human (patient) induced pluripotent stem cell lines, including the required experimental platforms for in-detail assessment of the derived human neuronal in-vitro network models.
Tasks and responsibilities
- You will be responsible for generating isogenic hIPSC cell lines and develop brain organoid model for mTORpathies.
- Designs and performs scientific research.
- Works in a team with PhD students, scientists and clinicians.
- Discusses, plans and performs research in a stimulating environment.
- Works in a highly international research group and is willing to travel to other institutes within Europe.
You have obtained an academic degree (MSc) in molecular life sciences, biomedical sciences or a related discipline. You also have a strong background in molecular and cellular neuroscience. Preferentially with basic experience in electrophysiology. You are highly motivated to work in an interdisciplinary environment at the cutting edge of science. You have e xperience with cell culture of primary neurons and induced pluripotent stem cells. In addition, excellent interpersonal and communication skills are essential, as is a willingness to work flexibly.
ConditionsWorking at Radboud university medical center means that you are ahead of the curve and working together on the healthcare of the future. But there is more. Our secondary terms of employment are impressive. These are fully tailored to you thanks to our Employment Conditions Selection Model. At Radboud university medical center, you will be given trust, and you will take the responsibility to handle everything together. We provide annual courses, both professional and personal.
- In addition to your monthly salary and an annual vacation allowance of 8%, you will receive an end-of-year bonus of 8.3%.
- If you work irregular hours, you will receive an allowance.
- As a full-time employee (36 hours per week), you are entitled to approximately 168 vacation hours (over 23 days) per year.
- Radboud university medical center pays 70% of the pension premium. You pay the rest of the premium with your gross salary.
- You get a discount on health insurance as well: you can take advantage of two group health insurance plans. UMC Zorgverzekering and CZ collectief.