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  3. PhD candidate 'Treating kidney disease by targeting a novel pathway that protects the kidney filter from injury'

PhD candidate 'Treating kidney disease by targeting a novel pathway that protects the kidney filter from injury'

In this project, we aim to characterize the endothelium-to-podocyte NO-mediated paracrine GFB signaling pathway, and test the promise of therapeutically …

28 dagen geleden

Arbeidsvoorwaarden

Standplaats:
Geert Grooteplein Zuid, Nijmegen, Gelderland
Dienstverband:
Tijdelijk contract / Tijdelijke opdracht
Uren per week:
36 - 36 uur
Salarisindicatie:
€ 2422 - € 3103 per maand
Opleidingsniveau:
WO

Functieomschrijving

In this project, we aim to characterize the endothelium-to-podocyte NO-mediated paracrine GFB signaling pathway, and test the promise of therapeutically targeting this pathway by repurposing already available drugs. The project entails both in vitro model systems (e.g. cell cultures and glomerulus-on-a-chip), as well as in vivo studies using specific kidney disease animal models as well as e.g. conditional podocyte-specific knockout animals.

Kidney diseases impact millions of people worldwide and can result in progressive chronic kidney disease (CKD), eventually necessitating renal replacement therapy through dialysis or kidney transplantation.

The glomerular filtration barrier (GFB) is a crucial part of the nephron, which is the functional unit of the kidney. GFB injury is a general characteristic of several kidney diseases and, if persistent, ultimately leads to loss of nephrons and kidney failure. Novel therapies to treat GFB injury or the ensuing progressive CKD have not emerged during the last decades. Thus, there is an unmet need for novel treatment targets in GFB injury, which can be translated into clinically applicable therapies at short term.

It is increasingly recognized that paracrine signaling between glomerular endothelial cells (GEnCs) and glomerular visceral epithelial cells (podocytes) is crucial to GFB integrity. We recently showed that nitric oxide (NO) produced by GEnCs can activate a podocyte signaling pathway crucial to maintain podocyte viability. Furthermore, GFB injury is associated with NO deficiency. This pathway can be therapeutically targeted by repurposing the class of sGC stimulator/activator drugs, mimicking the effect of NO on the podocyte.

Highlights of the current project:

  • Innovative research question based on a recently discovered signaling cascade.
  • An excellent and ambitious research environment and team, including basic scientists and clinicians.
  • Translation of basic research into novel therapeutic targets in kidney disease.
Tasks and responsibilities:
  • Plan, perform, analyze, and discuss experiments.
  • Contribute to scientific publications and present findings at scientific meetings.
  • Work together in a team in a stimulating environment.
  • Complete your project with a PhD thesis.

Functie-eisen

  • Master in Molecular Life Sciences, Molecular Biology, Biochemistry, or similar.
  • Experience in cell culture, molecular and cell biological techniques .
  • Affinity and/or experience in performing animal experiments (Article 9 is preferable).
  • Competencies we value: Enthusiasm, humor, high working standards, independent, team player, well-structured, communicative, reliable, ambitious.

Conditions

Upon commencement of employment we require a certificate of conduct (Verklaring Omtrent het Gedrag, VOG) and there will be, depending on the type of job, a screening based on the provided cv. Radboud university medical center's HR Department will apply for this certificate on your behalf.

Read more about the Radboudumc employment conditions and what our International Office can do for you when moving to the Netherlands.

Additional information

Applicants are requested to send a letter of application outlining their specific interest in the position and a curriculum vitae including 2 references.

All additional information about the vacancy can be obtained from Dr. Tom Nijenhuis, internist-nephrologist, +31 (0)24 361 47 61 or Prof. Dr. Johan van der Vlag, Professor in Immunology of Kidney Diseases and Transplantation, +31 (0)24 361 65 39. Use the Apply button to submit your application.

Please apply before 4 September 2020.
Recruitment agencies are asked not to respond to this job posting.

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