Ongeveer 22 uur geleden - NWO-institutenorganisatie - Nijmegen
During this project you will develop and exploit an experimental setup able to measure the spatial diffusion of photo-excited electron-hole pairs (excitons) …
Mass spectrometry, microbial cell factories, enzymes
The PhD candidate at the TU Delft will establish a proteomics platform for analysing post-translational protein modification networks in microbial cell factories. The facility will be equipped with state-of-the-art high-resolution mass spectrometry (MS) instrumentation, including the latest bioinformatics tools. Initial large-scale screening will channel into quantitative monitoring of enzymes from central metabolic pathways. Large-scale MS data will be integrated into other omics data such as metabolomics for further correlation with cell performance to determine the regulatory potential of a broad range of protein modifications in microbial cell factories. During the progress of this project, the candidate is expected to further develop methods for identification and quantification of post-translational modifications using mass spectrometry.
The protein analytics group at the TU Delft is looking for a highly motivated, technically skilled, and very ambitious PhD candidate with a strong background and experience in biotechnology, biochemistry, or a related chemical science. Preferably, the candidate also has good experience in chromatography, high-resolution mass spectrometry, and related computational approaches. The candidate must be eager to work in a multidisciplinary and dynamic setting with a focus on applied research in the field of microbial protein mass spectrometry. The position will be for a term of four years and is expected to result in a PhD. Good communication skills in English are required.
Delft University of Technology (the TU Delft) is a multifaceted institution offering education and carrying out research in the technical sciences at an internationally recognised level. Education, research and design are strongly oriented towards applicability. The TU Delft develops technologies for future generations, focusing on sustainability, safety and economic vitality. At the TU Delft you will work in an environment where technical sciences and society converge. The TU Delft comprises eight faculties, unique laboratories, research institutes and schools.
Innovation is crucial to fulfil the potential of industrial biotechnology for sustainable production of fuels, chemicals, materials, food, and feed. Similarly, scientific and technological advances in environmental biotechnology are needed to enable novel approaches to water purification, and ‘waste-to-product’ processes, thus contributing to a circular economy. Increased fundamental knowledge encompassing enzymes, micro-organisms, and processes is essential for progress in this field.
The Department of Biotechnology covers this research area and, based on new insights, selects, designs, and tests new biobased catalysts, micro-organisms, and processes.
The Protein MS research group is a highly dynamic team located within the Cell Systems Engineering section of the Department of Biotechnology at the TU Delft. The facility will be equipped with state-of-the-art LC and high-resolution MS equipment. The group performs research on novel methods for protein characterisation, quantification, and analysis of post-translational protein modifications, with a strong focus on microbial cell systems.