Ongeveer 16 uur geleden - Technische Universiteit Delft (TUD) - Delft
The PhD project is part of a European Network with altogether 11 PhD projects on cutting-edge, multidisciplinary research, see …
Understanding protein biogenesis
Chaperones are central to protein biogenesis, but their action at the ribosome remains poorly understood. A central complication is the highly dynamic nature of this process, which involves polypeptide synthesis, folding, chaperone binding, and ATP cycles, as well as a possible interplay between chaperones. New tools now allow us to directly monitor these dynamics. Recently it has become possible to detect protein conformational changes mediated by chaperones, as we have shown at the single-molecule level using optical tweezers for SecB, Trigger Factor, and Hsp70 (Science 2007, Nature 2013, Nature 2016). The recent integration of single-molecule fluorescence now allows us to visualise the composition of actively translating ribosome-chaperone-client complexes, pushing the envelope of technical possibilities. In this project, we use this novel approach together with ribosomal profiling in order to unravel the first steps of protein biogenesis in real time.
The position is intended as a full-time appointment. The formal employment and main activities will take place at AMOLF in Amsterdam. The student will participate in the TU Delft graduate school, and the PhD degree will be granted at the TU Delft.
We are looking for an outstanding experimental physicist or chemist with an interest in single-molecule biophysics and a strong drive to excel in a competitive, international environment. For a post-doctoral appointment you must have a PhD degree. Good communication skills in English are required.
Delft University of Technology (the TU Delft) is a multifaceted institution offering education and carrying out research in the technical sciences at an internationally recognised level. Education, research and design are strongly oriented towards applicability. The TU Delft develops technologies for future generations, focusing on sustainability, safety and economic vitality. At the TU Delft you will work in an environment where technical sciences and society converge. The TU Delft comprises eight faculties, unique laboratories, research institutes and schools. Applied Sciences AMOLF performs leading fundamental research on the physics of biomolecular systems and nanophotonics, two areas with key potential for technological innovations. The Institute contributes to knowledge transfer to industry and society and trains talented young researchers. AMOLF is located at Science Park Amsterdam, the Netherlands, and engages approximately 140 scientists and a support staff of 70. See also www.amolf.nl.