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  2. Technische Universiteit Delft (TUD)
  3. Postdoc position: Cryogenic superresolution fluorescence targeting for FIB milling in electron cryo-tomography

Postdoc position: Cryogenic superresolution fluorescence targeting for FIB milling in electron cryo-tomography

With transmission electron cryo-microscopes, the structure of proteins in their cellular context can be revealed almost at atomic detail. However, most …

26 dagen geleden

Arbeidsvoorwaarden

Standplaats:
Lorentzweg, Delft, Zuid-Holland
Dienstverband:
Tijdelijk contract / Tijdelijke opdracht
Uren per week:
38 - 38 uur
Salarisindicatie:
€ 2709 - € 4274 per maand
Opleidingsniveau:
Postdoctoraal

Functieomschrijving

With transmission electron cryo-microscopes, the structure of proteins in their cellular context can be revealed almost at atomic detail. However, most applications are currently focused only on purified proteins or on highly abundant protein complexes in situ. The problem is that for cellular samples, the target proteins need to be contained precisely in a 100-200 nm thin slice cut out of the ~1000 µm3 cellular volume using a focused ion beam while these proteins themselves are invisible. To address this challenge, within a large international consortium and together with our spin-off partner Delmic, we are developing a novel cryogenic microscope that integrates photon, electron, and ion beams. With fluorescence light microscopy, the target proteins will be visualized and tracked while the sample is moved into position for focused ion beam milling. We aim to determine the 3D position of target proteins with high enough accuracy to mill the 100-200 nm slice containing precisely these proteins.
To strengthen our project team, we are now looking for a postdoctoral candidate. You will work on the interface between engineering and biological applications in both the Hoogenboom lab at the Imaging Physics department and the Jakobi lab at the Bionanoscience department. A PhD candidate has already started on the project and a first prototype of the integrated fluorescence-electron-ion beam microscope is currently being tested. Your main focus will be on the implementation of 3D fluorescence localization in the cryo-microscope and/or its application in targeted FIB milling of lamellae in cryo-frozen materials, and their further analysis with electron cryo-microscopy. You will further collaborate with the industrial project team and PhD students and Postdocs in both the Hoogenboom and Jakobi labs. As a postdoctoral candidate, we further expect you to take a supervising role on the scientific part of this project.

Functie-eisen

As a successful candidate you hold a PhD in physics, applied physics, biophysics, biomedical engineering, or similar. Experience in either the development or implementation of (cryo-) superresolution fluorescence microscopy and/or FIB milling on cryo-frozen materials is strongly preferred. You have a hands-on mentality, shown ability to work in a strongly multi-disciplinary and international environment, and you have an affinity to work with, and improve, new technology. You have an excellent command of the English language and can easily and effectively communicate with academic and industrial collaborators alike.

Conditions

Salary and benefits are in accordance with the Collective Labour Agreement for Dutch Universities. The TU Delft offers a customisable compensation package, a discount on health insurance and sport memberships, and a monthly work costs contribution. Flexible work schedules can be arranged. For international applicants we offer the Coming to Delft Service and Partner Career Advice to assist you with your relocation. An International Children's Centre offers childcare and there is an international primary school.

Additional information

For information about this vacancy, you can contact Dr.ir. J.P. Hoogenboom, email: j.p.hoogenboom@tudelft.nl, tel: +31(0)15 27 85920.

Send your application with CV and motivation letter by email to Anjella van Vliet, secretary MInT, email: j.t.vanvliet@tudelft.nl.

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