PhD PCC/BIC

A PhD candidate for the project Coacervate protein carriers with application-dependent stability and delivery properties. Micelles composed of polyelectrolyte complexes (complex coacervate core micelles, C3Ms) are promising structures for protein protection and controlled delivery. They ...

11 dagen geleden

Arbeidsvoorwaarden

Standplaats:
Bornsesteeg, Wageningen, Gelderland
Dienstverband:
Tijdelijk contract / Tijdelijke opdracht
Uren per week:
38 - 38 uur
Opleidingsniveau:
WO

Functieomschrijving

A PhD candidate for the project Coacervate protein carriers with application-dependent stability and delivery properties. Micelles composed of polyelectrolyte complexes (complex coacervate core micelles, C3Ms) are promising structures for protein protection and controlled delivery. They are simple to prepare: encapsulation of protein molecules can be achieved by mixing protein solutions with oppositely charged diblock copolymer solutions at stoichiometric charge ratio. The inner core of the C3M has a high loading capacity: many protein molecules can be incorporated into one micelle. Unfortunately, practical application is hampered by the low stability and high exchange dynamics of protein-containing C3Ms, due to the generally low charge density of protein molecules. In this project, we will take concrete steps towards application of C3Ms as protein carriers and delivery systems. We will use biocompatible block copolymers for encapsulation of two model proteins, i.e. the enzymes proline dehydrogenase and laccase, representative for a broad spectrum of enzyme types. Several strategies will be tested: 1) to enhance and tune the stability of the protein-polymer complexes, state-of-the-art bio-conjugation chemistry tools can be used to provide the enzymes with additional charged polypeptide sequences of variable length, and 2) crosslinking of the polyelectrolyte chains in the core can be used to inhibit protein exchange with the solution. The encapsulation efficiency, formation and decomposition kinetics, exchange dynamics (e.g., under physiological solutions) and the effect of packaging on the activity of the enzymes will be determined. Finally, we will explore a number of release mechanisms. On the basis of the obtained results, it will be possible to design C3M protein carriers of which the stability and dynamics are tuned to specific applications.

Functie-eisen

We are looking for a highly motivated, ambitious and creative candidate with a background in physical chemistry, colloid science, and biochemistry. Experience in fluorescence and bio-conjugation chemistry techniques is considered as a strong recommendation.

Conditions

We offer you a temporary position for a period of 1.5 years with extension of 2.5 years after successful evaluation. Gross salary per month €2.222,00 in the first year rising up to €2.840,00 per month in the fourth year.

We not only offer a competitive salary but also good (study) leave and a pension of the ABP Pension Fund.

Additional information

This is a joined project of the chair groups Physical Chemistry & Soft Matter and the Laboratory of Biochemistry of Wageningen University. For more information on the project, please contact Dr. Mieke Kleijn, mieke.kleijn@wur.nl, or Dr. Jan Willem Borst, janwillem.borst@wur.nl. For more information about the contractual aspects, please contact Marjolijn Steffens, HR advisor, telephone number +31 317 489882 .

Interested?
You can apply online at the career site of Wageningen UR until October 1, 2017.

Bedrijfsomschrijving

Physical Chemistry and Soft Matter (PCC) at Wageningen University is interested in phenomena at the nanoscale, where creative chemistry is essential, complex physics is a rule rather than an exception and biology comes to life. The laboratory works on Soft Matter in the broadest sense of the word, combining experiment and theory, chemistry and physics and fundamental and applied science. The laboratory has a large range of state-of-the art equipment. See www.pcc.wur.nl

The Laboratory of Biochemistry (BIC) at Wageningen University focusses on cellular biochemistry. Molecular genetic, bio-informatic and spectroscopic approaches are used to link protein structure and function with protein activity in a cell and in multi-cellular organisms (see www.bic.wur.nl/UK/). Within this laboratory, Dr. Borst leads the Biomolecular Imaging group and is affiliated in the Microspectroscopy research facility (see www.wur.nl/en/Expertise-Services/Facilities/Microspectroscopy-Research-Facility.htm)

Wageningen University & Research
Delivering a substantial contribution to the quality of life. That's our focus - each and every day. Within our domain, healthy food and living environment, we search for answers to issues affecting society - such as sustainable food production, climate change and alternative energy. Of course, we don't do this alone. Every day, 5000 people work on 'the quality of life', turning ideas into reality, on a global scale.

Could you be one of these people? We give you the space you need.

For further information about working at Wageningen University & Research, take a look at www.wur.nl/en/Jobs.htm

Acquisition regarding this vacancy is not appreciated.

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